FIGURE 2.

Running attenuated age‐dependent weight gain and fat accumulation across apolipoprotein E (APOE) genotypes. A‐B, Expected age‐dependent weight gain from 1 to 12 months in females (A, n = 9–15) and males (B, n = 11–15). C‐D, Running mice weighed significantly less at 12 months in both females (C) and males (D). E‐F, Running significantly attenuated age‐dependent weight gain (the difference in body weight from 1 to 12 months) in both females (E) and males (F). G‐I, Significant effect of running on the change in lean mass (G), fat mass (H), and fat percentage (I) between 6 and 11 months, with an overall reduction in running mice compared to sedentary mice across all APOE genotypes in females. J‐L, Running had a significant reduction on the change in lean mass (J) and fat mass (K) between 6 and 11 months, but no change in fat percentage (L) in male mice. Data presented as mean ± standard error of the mean, two‐way analysis of variance performed for APOE genotype (significant marked above “Sed” column, indicating an effect of APOE genotype), Running (significance marked above “Run” column, indicating an effect of running), and the interaction between APOE genotype:Running (significance marked to the right of the graph). Bonferroni's multiple comparisons performed for within‐genotype running effects (significance marked in smaller stars directly to the right of the run column, within graph limits, in the color of the genotype). *P < .05, **P < .01, ***P < .001, ****P < .0001