Fig. 3.

Interaction rewiring in RAS/RAF pathway. A A network diagram of differential interactions in the RTK/MAP kinase signaling cascade caused by mutations on KRAS, NRAS, and BRAF. B KRAS gain of function mutant caused loss of NRAS-RAF1 interaction and NRAS mutant caused loss of KRAS-RAF interaction. C RAF1 is required for transduction signal to MEK/ERK signal when BRAF is wildtype, while BRAF mutant does not require RAF1 and led to loss of RAF1 interaction with BRAF and the downstream pathway. D, E Scatter plots of interactions between RAF1 and two RAS proteins