Fig. 7.

ERK signal involves in CXCL1-mediated malignant phenotypes in HeLa cells. A Expression of ERK, p-ERK, Cyclin D1 and Bax at protein levels from HeLa cells overexpressing CXCL1 and their mock controls were assessed by western blotting. B The protein level of the p-ERK was quantitated and normalized to total ERK from western blotting. C Protein expression of ERK, Cyclin D1 and BAX were statistically analyzed from western blotting, and their bands were normalized to β-actin. D The proliferation inhibition rate of ERK inhibitor PD98059 on HeLa cells overexpressing CXCL1 and their mock controls was determined by CCK-8 assay. E–F The effect of ERK inhibitor PD98059 on migration inhibition rate of HeLa cells overexpressing CXCL1 and their mock controls was assessed by transwell analysis. *P < 0.05, **P < 0.01