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. 2022 Jun 28;17:47. doi: 10.1186/s13024-022-00545-9

Fig. 4.

Fig. 4

Impaired uptake of fAβ and lysosomal acidification in 6 month-old 5xFAD;Cx3cr1−/− mice. (A) Representative flow cytometry plots identifying subpopulations of methoxy-X04+CD11b+CD45low and methoxy-X04+CD11b+CD45high microglia and (B) mean fluorescence intensities (MFI) for Lysotracker Deep-Red™ (-DR) to assess the acidification of endolytic compartments of these individual subpopulations. Data representative of flow cytometry experiments done using n = 5–6 mice (males and females) of 6 month-old 5xFAD;Cx3cr1+/+ and 5xFAD;Cx3cr1−/− mice. Quantification of (C) proportion of methoxy-X04+CD11b+CD45low and methoxy-X04+CD11b+CD45high microglia and (D) Lysotracker-DR MFIs for methoxy-X04low and methoxy-X04high microglia within the CD11b+CD45low and CD11b+CD45high subsets in 6 month-old 5xFAD;Cx3cr1+/+ (black bars) and 5xFAD;Cx3cr1.−/− (grey bars) mice. Data in C,D represents means calculated using n = 5–6 mice (males and females) per genotype. Error bars represent SEM. All animals were processed and analyzed in a single experiment to enable MFI comparisons across genotypes and individual mice. Statistics for data in C,D was done using two-way ANOVA with Tukey’s multiple comparison tests. *p < 0.05, **p < 0.001, ***p < 0.0001, ****p < 0.00001