Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 28;17:47. doi: 10.1186/s13024-022-00545-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 7 — Aggravated synaptic dysfunction, neuronal loss and cognitive decline in the absence of Cx3cr1. (A) Representative western blots to assess the levels of pre-synaptic proteins—Synaptic Vesicle Protein-2 (SV2a), Synaptophysin and Homer, and post-synaptic proteins—Post-Synaptic Density-19 (PSD19) and NMDA Receptor subunit 1 (NR1) in cortical lysates of 6 month-old 5xFAD;Cx3cr1^+/+ and 5xFAD;Cx3cr1^−/− mice and their genotype matched controls. Blots representative of n = 6 (3 females, 3 males) per genotype. (B) Visualization and quantitation of NeuN⁺ neuronal abundance in the subiculum of 4- and 6 month-old 5xFAD;Cx3cr1^+/+ (black bars) and 5xFAD;Cx3cr1^−/− mice (grey bars). Images representative of NeuN⁺ neurons in subiculum of 6 month-old cohorts. Scale bars = 100 µm. Histological analyses and related quantification performed using multiple, serial sections from n = 6 mice (3 females, 3 males), of each genotype and at each time-point. Error bars represent SEM. Statistical analysis done using Two-way ANOVA (p^int = ns) followed by Tukey’s post-hoc tests. ***p = 0.0002, **p < 0.005. Densitometric analysis to quantify fold changes in (C) pre-synaptic proteins and (D) post-synaptic markers in cortical lysates of B6;Cx3cr1^+/+ (white bars), B6;Cx3cr1^−/− (light grey bars), 5xFAD;Cx3cr1^+/+ (black bars) and 5xFAD;Cx3cr1^−/− (dark grey bars) mice 5xFAD;Cx3cr1^−/− (dark grey bars). Fold changes were calculated using blots from n = 6 animals (3 females, 3 males) of each genotype run on the same gel to enable comparisons. Statistical analyses done using One-way ANOVA followed by Brown-Forsythe and Welch’s post-hoc tests. *p^adj < 0.05, **p^adj < 0.001, ***p^adj < 0.0001, ****p^adj < 0.00001. (E) Y-maze based evaluation of working memory in 6 month-old B6;Cx3cr1^+/+ mice (white bars), B6;Cx3cr1^−/− (light grey bars), 5xFAD;Cx3cr1^+/+ (black bars) and 5xFAD; Cx3cr1^−/− (dark grey bars). Behavioral testing done using ~ n = 10 animals (5 females, 5 males) per genotype. Statistical analysis done using One-way ANOVA followed by Brown-Forsythe and Welch’s post-hoc tests. *p^adj = 0.02, ***p.^adj < 0.0001, ns = not significant