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. 2022 Apr 19;20(7):1346–1362. doi: 10.1111/pbi.13812

Figure 5.

Figure 5

OsHIPL1 altering the contents of ABA and expressions of ABA signalling genes OsABIs during seed germination. (a) The contents of ABA in germinated seeds of IR26, NIL‐qsv3 and Oshipl1 mutants. (b, c) Transcription levels of ABA biosynthesis genes (OsZEP, OsNCED1, OsNCED3, OsNCED4 and OsNCED5) (b) and ABA degradation genes (OsABA8ox1 and OsABA8ox3) (c) among IR26, Oshipl1 mutants and NIL‐qsv3 line at the 12 h seeds after imbibition. The relative expression levels were represented by fold change relative to the expression levels of IR26 at 12h after imbibition. (d–f) Comparison of OsABI3 (d), OsABI4 (e) and OsABI5 (f) expression in germinated seeds among NIL‐qsv3, Oshipl1 mutants and IR26 (control) during seed germination. The relative expression levels were represented by fold change relative to the expression levels of IR26 at 12 h after imbibition. (g–i) Expressions of OsABI3 (g), OsABI4 (h) and OsABI5 (i) in germinated seeds were induced at ABA treatments in IR26 and NIL‐qsv3 using the RT‐qPCR approach. The relative expression levels were represented by fold change relative to the expression levels of IR26 without ABA. The expression of OsHIPL1 was normalized to that of OsActin gene control.