Table 1.
Putative WRI1 targets
| Line | log2FC_Greenhouse | log2FC_Field | AW site | PCC ATWRI1 | Top Arabidopsis BLASTP Hit | ATH hit annotations | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WD‐1 | WD‐2 | WD‐1 | WD‐2 | WD‐1 | WD‐2 | WD‐1 | WD‐2 | WD‐1 | WD‐1 | WD‐1 | W‐1 | W‐1 | W‐1 | |||||
| Gene ID | 24 DAP | 27 DAP | 30 DAP | 33 DAP | R5 | R5/6 | R6 | R5 | R5/6 | R6 | ||||||||
| Glyma.16G173100 | 1.5 | 1.3 | 2.1 | 2.4 | 2.1 | 2.3 | 2.2 | 1.7 | 2.1 | 2.0 | 526 | 0.99 | AT5G52920 | Pyruvate kinase beta subunit 1 | ||||
| Glyma.18G023300 | 1.2 | 1.1 | 1.9 | 2.1 | 1.8 | 2.0 | 1.9 | 1.4 | 1.7 | 1.6 | 913 | 0.99 | AT3G56130 | Biotin/lipoyl attachment domain‐containing protein | ||||
| Glyma.05G195500 | 4.4 | 4.5 | 4.1 | 4.7 | 4.5 | 4.8 | 4.7 | 4.3 | 2.5 | 4.0 | 2.5 | 4.0 | 1089 | 0.98 | AT5G64440 | Fatty acid amide hydrolase | ||
| Glyma.03G168700 | 1.4 | 1.2 | 1.4 | 1.7 | 1.3 | 1.4 | 1.8 | 1.1 | 1.7 | 1.9 | 1896 | 0.98 | AT5G43940 | Alcohol dehydrogenase 2 | ||||
| Glyma.17G231400 | 1.3 | 1.1 | 1.8 | 2.0 | 1.7 | 1.7 | 1.8 | 1.1 | 1.8 | 1.9 | 1608 | 0.97 | AT2G34590 | Pyruvate dehydrogenase 1b | ||||
| Glyma.08G024700 | 2.0 | 2.1 | 4.9 | 1.6 | 3.5 | 2.7 | 4.8 | 1.8 | 1.8 | 1876 | 0.97 | AT5G46290 | Ketoacyl‐ACP Synthase I | |||||
| Glyma.18G211400 | 1.2 | 1.8 | 1.8 | 1.8 | 1.7 | 1.8 | 1.2 | 1.6 | 1.6 | 2 | 0.96 | AT1G62640 | Ketoacyl‐ACP synthase III | |||||
| Glyma.07G033000 | 1.0 | 1.2 | 1.2 | 1.6 | 1.3 | 1.4 | 2.4 | 2.2 | 1587 | 0.96 | AT1G80280 | Alpha/beta‐hydrolases | ||||||
| Glyma.20G122500 | 1.1 | 1.1 | 1.5 | 1.6 | 1.3 | 1.4 | 1.8 | 1.2 | 1.7 | 1.6 | 586 | 0.96 | AT2G01140 | Fructose bisphosphate aldolase 3 | ||||
| Glyma.08G179900 | 1.4 | 1.1 | 1.9 | 1.9 | 2.1 | 2.2 | 2.3 | 1.6 | 1.7 | 1.7 | 306 | 0.96 | AT2G22230 | Hydroxyacyl‐ACP dehydrase | ||||
| Glyma.01G081900 | 3.1 | 2.6 | 5.0 | 4.9 | 5.0 | 4.8 | 6.0 | 5.3 | 1.7 | 4.0 | 1.8 | 4.1 | 1552 | 0.95 | AT3G25860 | Pyruvate dehydrogenase E2 | ||
| Glyma.17G015500 | 1.9 | 1.6 | 2.9 | 2.8 | 2.9 | 2.8 | 3.2 | 2.2 | 1.1 | 3.9 | 1.1 | 4.1 | 244 | 0.95 | AT1G61800 | Glucose 6 phosphate/phosphate translocator 2 | ||
| Glyma.15G276300 | 1.6 | 1.8 | 1.4 | 1.6 | 1.6 | 1.1 | 1.2 | 1.2 | 405 | 0.95 | AT5G13710 | Sterol methyltransferase 1 | ||||||
| Glyma.06G214800 | 1.3 | 1.1 | 1.7 | 1.6 | 1.8 | 1.7 | 3.0 | 3.0 | 1890 | 0.95 | AT2G26640 | Ketoacyl‐CoA synthase 11 | ||||||
| Glyma.13G152500 | 1.0 | 1.3 | 1.5 | 1.1 | 1.3 | 1.5 | 1.2 | 1.6 | 1.5 | 1971 | 0.95 | AT5G17310 | UDP‐glucose pyrophosphorylase 2 | |||||
| Glyma.03G092700 | 1.2 | 1.3 | 1.3 | 1.5 | 1.4 | 1.1 | 1.4 | 1.6 | 58 | 0.94 | AT1G79530 | Glyceraldehyde‐3‐phosphate dehydrogenase 1 | ||||||
| Glyma.05G000900 | 1.1 | 1.9 | 1.8 | 1.7 | 1.6 | 1.8 | 1.2 | 1.7 | 1.6 | 1613 | 0.94 | AT3G12110 | Actin 11 | |||||
| Glyma.04G149300 | 1.6 | 1.4 | 2.3 | 2.3 | 2.7 | 3.1 | 2.9 | 2.7 | 104 | 0.94 | AT2G26640 | Ketoacyl‐CoA synthase 11 | ||||||
| Glyma.08G003100 | 3.8 | 3.9 | 3.5 | 4.3 | 3.9 | 4.3 | 4.6 | 4.6 | 1.6 | 3.6 | 3.0 | 4.7 | 936 | 0.94 | AT5G64440 | Fatty acid amide hydrolase | ||
| Glyma.11G233700 | 1.1 | 1.3 | 1.0 | 1.2 | 1.3 | 1.2 | 1.5 | 800 | 0.93 | AT3G56130 | Biotin/lipoyl attachment domain‐containing protein | |||||||
| Glyma.10G215400 | 1.1 | 1.8 | 1.7 | 2.0 | 1.9 | 1.8 | 1.2 | 1.6 | 1.6 | 121 | 0.93 | AT1G34430 | Pyruvate dehydrogenase E2 | |||||
| Glyma.12G135300 | 3.6 | 3.3 | 3.9 | 4.1 | 4.2 | 4.4 | 4.2 | 3.8 | 1.7 | 5.0 | 2.2 | 4.9 | 1921 | 0.92 | AT3G28345 | ABC transporter | ||
| Glyma.01G190600 | 1.2 | 1.3 | 1.8 | 1.7 | 1.8 | 2.4 | 1909 | 0.92 | AT2G14960 | Auxin‐responsive GH3 family protein | ||||||||
| Glyma.04G151600 | 1.4 | 1.3 | 1.5 | 2.0 | 1.6 | 2.0 | 2.2 | 1.8 | 2.3 | 2.2 | 163 | 0.92 | AT1G08510 | Acyl‐ACP thioesterase B | ||||
| Glyma.20G114500 | 1.2 | 2.0 | 1.7 | 2.0 | 1.7 | 2.0 | 1.1 | 1.6 | 1.8 | 1736 | 0.92 | AT3G25860 | Pyruvate dehydrogenase E2 | |||||
| Glyma.15G178400 | 1.0 | 1.3 | 1.7 | 1.4 | 1.4 | 1.3 | 1.6 | 1.5 | 1895 | 0.92 | AT1G54100 | Aldehyde dehydrogenase 7B4 | ||||||
| Glyma.08G164600 | 1.5 | 1.5 | 2.2 | 2.1 | 2.5 | 2.7 | 3.1 | 2.0 | 2.9 | 3.4 | 1834 | 0.91 | AT3G04940 | Cysteine synthase D1 | ||||
| Glyma.15G182900 | 4.7 | 5.4 | 5.8 | 7.1 | 5.1 | 6.3 | 5.7 | 5.8 | 3.5 | 5.6 | 5.9 | 95 | 0.91 | AT1G53920 | GDSL‐motif lipase 5 | |||
| Glyma.05G026300 | 1.0 | 1.2 | 1.0 | 1.3 | 1.2 | 1.4 | 1.0 | 1.1 | 437 | 0.90 | AT3G47520 | NAD dependent malate dehydrogenase | ||||||
| Glyma.06G112900 | 1.2 | 1.0 | 1.4 | 1.3 | 1.4 | 1.1 | 1.2 | 1379 | 0.90 | AT1G77590 | Long chain acyl‐CoA synthetase 9 | |||||||
| Glyma.15G183000 | 1.5 | 1.7 | 3.1 | 3.9 | 2.7 | 3.8 | 3.5 | 3.5 | 3.9 | 1.5 | 4.3 | 75 | 0.90 | AT1G53920 | GDSL‐motif lipase 5 | |||
| Glyma.07G268100 | 1.0 | 1.2 | 1.4 | 1.2 | 1.2 | 1.3 | 1.3 | 1.4 | 1981 | 0.90 | AT5G47670 | LEC1‐LIKE, NF‐YB6,nuclear factor Y, | ||||||
| Glyma.06G087100 | 1.4 | 1.0 | 1.6 | 1.9 | 1.4 | 1.7 | 2.1 | 1.6 | 2.3 | 2.7 | 495 | 0.89 | AT5G11650 | Monoacylglycerol lipase | ||||
| Glyma.19G206100 | 3.1 | 2.6 | 2.9 | 3.3 | 3.0 | 3.3 | 3.8 | 3.6 | 2.2 | 3.9 | 2.0 | 3.6 | 211 | 0.89 | AT5G62000 | Auxin response factor 2 | ||
| Glyma.13G223800 | 2.4 | 2.4 | 2.8 | 2.5 | 2.4 | 1.9 | 2.2 | 1.3 | 2.5 | 4.1 | 2.9 | 4.5 | 687 | 0.89 | AT1G69850 | Nitrate transporter, ABA importer transporter 1 | ||
| Glyma.09G074000 | 5.2 | 5.9 | 5.3 | 6.7 | 5.1 | 6.4 | 5.8 | 6.4 | 4.4 | 4.9 | 1936 | 0.89 | AT1G53920 | GDSL‐motif lipase 5 | ||||
| Glyma.08G159500 | 1.0 | 1.9 | 1.5 | 2.0 | 2.0 | 2.2 | 1.8 | 1.7 | 1.9 | 1814 | 0.88 | AT1G05590 | Beta‐hexosaminidase 2 | |||||
| Glyma.06G312200 | 1.3 | 1.5 | 2.0 | 2.4 | 2.0 | 2.3 | 1.1 | 2.2 | 2.5 | 483 | 0.87 | AT1G65440 | Global transcription factor group B1 | |||||
| Glyma.07G027000 | 1.2 | 1.7 | 2.3 | 1.5 | 2.2 | 2.4 | 785 | 0.87 | AT1G80580 | Ethylene‐responsive transcription factor | ||||||||
| Glyma.01G198700 | 1.3 | 2.0 | 1.8 | 1.6 | 1.8 | 1.8 | 1.2 | 1.4 | 1.8 | 124 | 0.86 | AT4G17810 | C2H2 domain regulatory protein | |||||
Only genes with a significantly different expression and a |log2(Fold Change)| ≥ 1 with respect to the WT were included. PCC, Pearson correlation coefficient. DAP: days after pollination. WD‐1 and ‐2: independent transgenic events expressing AtWRI1 and ATDGAT1. W‐1 event expressing only AtWRI1 (Vogel et al., 2019).