TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| E. coli HB2151 | Δlac-pro ara Nalr thi F′ (proAB lacIq lacZΔM15) | 9 |
| E. coli XL1-Blue | endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 lac F′(proAB lacIqlacZΔM15 Tn10) | 8 |
| Plasmids | ||
| pCANTAB-5E | Apr; vector for periplasmic production of scFv's with E-tag epitope at C terminus | Pharmacia |
| pCANTAB-5Ehis | Apr; pCANTAB-5E with His6 tag incorporated before E-tag | This work |
| p6AC3g3 | Apr; scFv 6AC3 cloned between SfiI-NotI sites of pCANTAB-5Ehis | This work |
| pB4g3 | Apr; scFv B4 cloned between SfiI-NotI sites of pCANTAB-5Ehis | This work |
| pVDL9.3 | Cmr; production of HlyB and HlyD transporters | 35 |
| pLG612-1B | Apr; 23-kDa C-terminal domain of HlyA | 35 |
| pLG612-1SD | Apr; 23-kDa C-terminal domain of HlyA with Shine-Dalgarno sequence and NcoI site | 35 |
| pEHlyA | Apr; same as pLG612-1B with E-tag epitope incorporated at the 23-kDa C domain of HlyA | This work |
| pEHlyA1SD | Apr; same as pLG612-1SD with E-tag epitope incorporated at the 23-kDa C domain of HlyA | This work |
| pEHlyA2-SD | Apr; same as pEHlyA1SD with polylinker for cloning of scFv's in frame with E-tagged ′hlyA | This work |
| p6AC3HLYA | Apr; scFv 6AC3 cloned between NcoI and XmaI sites in pEHlyA2-SD | This work |
a
Apr, ampicillin resistant; Cmr, chloramphenicol resistant.