FIG 3.

Percent coverage of sequencing reads of enriched T. pallidum Nichols spiked samples. Treponemal reads with at least 1 read mapped per site (1×) against the T. pallidum subsp. pallidum Nichols reference genome (GenBank version number NC_000919.1) and percent coverage of the T. pallidum genome are shown. (A) Sequencing reads of samples enriched using the NEB+MDA method. (B) Sequencing reads of samples enriched using SWGA. All samples were sequenced using the Illumina NovaSeq 6000 platform. Error bars represent the standard error between the mapped reads derived from three replicate enriched Nichols samples.