FIG 5.

SWGA primer set validation. (A) T. pallidum gDNA copies/μL for the Nichols spiked sample (1:100 diluted) enriched with each SWGA primer set (1 to 12). (B) Relative percent T. pallidum DNA for the Nichols spiked sample (1:100 dilution) enriched with each SWGA primer set. Percent T. pallidum DNA was calculated based on the input DNA concentration and gDNA copies/μL for the Nichols mock samples post-SWGA enrichment. The spiked sample contained purified human gDNA, and the T. pallidum genome copies were derived from qPCR-measured T. pallidum polA copies/μL of DNA extract. The input T. pallidum gDNA copies/μL of DNA is displayed as nonenriched. The y axis is log₁₀-scaled in each panel for depiction of the relative percent T. pallidum post-enrichment with each primer set. Error bars represent the standard error among three replicate Nichols samples.