FIG 4.

Role of membrane localization and phosphatase activity in TgPRL function. The Δprl knockout strain was transfected with mutant versions (C339S and C401A) of the complementation plasmid shown in Fig. 2C. (A) Intracellular parasites expressing the C339S or C401A mutant TgPRL were stained to determine the localization of exogenous mutant TgPRL. As expected, the C339S mutant correctly associates with the plasma membrane, and the C401A mutant is mislocalized to the cytoplasm of the parasite. (B) Representative images of the plaque assay of corresponding strains. The chart on the right is the quantification of the plaque area relative to the parental strain. For all data, n = 3 biological replicates × 3 experimental replicates, and the P value was estimated by two-tailed Student's t test. Error bars show standard deviations. ***, P < 0.0001.