TABLE 1.
List of proteins identified in HEVs and YEVs related to biological processes that are enriched in HEV protein cargo and to virulence
| Biological process | No. of proteins identified: name(s) or ORF(s)a |
Total no. in: |
|||
|---|---|---|---|---|---|
| In both YEVs and HEVs | Exclusively in: |
||||
| YEVs | HEVs | YEVs | HEVs | ||
| Cellular processes | |||||
| Proteasome | 2: Pre5 (α6), Orf19.1785 | 31: Scl1 (α1), Pre8 (α2), Pre9 (α3), Pup2 (α5), Prs1/Pre10 (α7), Pre3 (β1), Pup1 (β2), Pup3 (β3), Pre1 (β4), Pre2 (β5), Pre4 (β7), Rpt1, Rpt2, Rpt4, Rpt5, Rpt6, Rpn1, Rpn2, Rpn3, Rpn5, Rpn6, Rpn7, Rpn8, Rpn11, Ubp6, Ubc4, Ecm29, Hsm3, Orf19.2278, Orf19.6604, Pr26 | 33 | ||
| Translation factors (initiation, elongation, and release) | 4: Ded81, Anb1, Tif, Tef2b | 13: Fun12, Sui2, Eif4e, Tif11, Tif5, Nip1, Prt1, Sui1, Sui3, Gcd2, Gcd11, Ria1,b Erf1c | 4 | 17 | |
| tRNA synthetases and ligases | 1: Orf19.6701d | 14: Grs, Dps1-1, Gln4, Mes1, Orf19.4931, Wrs1, Vas1, Tys1, Ths1, Hts1, Mes1, Frs1, Frs2, Gus1d | 1 | 15 | |
| Ribosomal proteins | 4: Asc1, Rpl12, Rpl14, Rpl10a | 68: Rps18, Rps3, Rpl23a, Rpl6, Rps14b, Rpl3, Rpl20b, Rps8a, Rps26a, Rps27, Rpl10, Rps15, Rps5, Rps6a, Rps9b, Rpp0, Rps20, Rpl9b, Rpl4b, Rps22a, Rpl11, Rpl16a, Rpl19a, Rpl28, Rps24, Rpl38, Rpl24a, Rps17b, Rpl21a, Rps25b, Rps7a, Rpl15a, Rpl18, Rpl17b, Rpl13, Orf19.3341, Rps23a, Rpl5, Rpl7, Rps16a, Rps1, Orf19.4149.1, Rps13, Rpl27a, Orf19.2478.1, Rpl30, Rpl39, Rpl32, Rps19a, Orf19.3572.3, Orf19.3690.2, Rpl25, Rpl2, Rpl43a, Rpl35, Rps12, Rps21b, Rpl42, Rpp2a, Rps28b, Rpp2b, Rpl37b, Orf19.6220.4, Orf19.828, Orf19.512, Orf19.3778, Orf19.3559, Orf19.5698 | 4 | 72 | |
| Purine and pyrimidine biosynthesis | 4: Ade13, Ade17, Ade 6, Ado1 | 17: Ade1, Ade2, Ade4, Ade5, Ade12, Imh3, Gua1, Cpa1, Cpa2, Ura1, Ura2, Ura3, Ura4, Ura5, Ura6, Ura7, Prs1 | 4 | 21 | |
| Amino acid biosynthesis | 20: Hom6, Met6, Met15, Sah1, Sam2, Shm2, Cys3, Idp1, Idp2, Car2, His7, Asn1, Aro4, Aro8, Leu2, His1, Ser33, Arg1, Lys9, Ilv5 | 1: Hom2 | 46: Aro3, Aro9, Pro1, Pro2, Pro3, Lys1, Lys2, Lys4, Lys12, Lys21, Lys22, Arg3, Arg4, Arg5,6, Arg8, Trp2, Trp3, Trp4, Trp5 Cys4, Ser1, Ser2, His4, His5, His7, Tyr1, Prs1, Orf19.6306, Met2, Met3, Met10, Met13, Met14, Met16, Met18, Met13, Ilv1, Ilv2, Ilv3, Ilv6, Hom3, Sam51, Shm1, Leu1, Leu4, Leu42 | 21 | 66 |
| Ergosterol biosynthesis | 3: Erg10, Erg13, Erg20 | 2: Fmp45, Gcy1 | 11: Erg1, Erg3, Erg4, Erg5, Erg6, Erg9, Erg11, Ncp1, Erg26, Erg27, Hmg1 | 5 | 14 |
| Required for resistance to toxic ergosterol analog | 5: Car2, Dag7, Orf19.2047, Mnn23, Ypt31 | 1: Sap3 | 4: Amo2, Apl2, Nat2, Vid27 | 5 | 9 |
| Induced by azole treatment or linked to azole resistance | 52: Ach1, Aco1, Acs1, Adh1, Ado1, Ahp1, Ald5, Atp1, Cat1, Cht2, Dag7, Dak2, Ecm33, Eng1, Erg10, Ero1, Fba1, Fdh1, Fet34, Fma1, Gdh3, Glk1, Glx3, Gpm1, Grp2, Hhf1, Hsp70, Hsp90, Hxk2, Mcr1, Mid1, Mp65, Msi3, Orf19.1765, Orf19.1766, Orf19.7306, Pbr1, Pck1, Pdc11, Pet9, Pga52, Phr2, Plb3, Png2, Prx1, Pyc2, Rbt1, Rhd3, Sah1, Sur7, Tos1, Xyl2 | 7: Ade17, Als2, Bat22, Hom2, Orf19.4211, Pir1, Plb1 | 75: Asm3, Cdc3, Cka1, Cka2, Cmp1, Csh1, Cyb5, Ece1, Ecm331, Ena21, Erg11, Erg3, Erg4, Erg6, Erg9, Fas1, Fas2, Frp3, Fum12, Gal1, Gal10, Gal7, Glc3, Gph1, Gst2, Hgt7, Hsp21, Hym1, Hyr1, Ifd6, Ife2, Lsc1, Lys21, Lys22, Met13, Met3, Mir1, Mis11, Ncp1, Ole1, Op4, Orf19.1239, Orf19.2269, Orf19.2286, Orf19.2452, Orf19.2473, Orf19.3475, Orf19.3932, Orf19.4476, Orf19.6553, Orf19.6554, Orf19.7310, Orf19.7459, Orf19.851, Pda1, Pdb1, Pdr16, Pfk1, Pfk2, Plb5, Pma1, Por1, Rct1, Rnr21, Rpl35, Scs7, Sds24, Snf1, Snz1, Svf1, Tub2, Ucf1, Vma8, Zpr1 | 59 | 127 |
| Virulence related | |||||
| Phospholipases | 3: Plb4.5, Plb2, Plb3 | 1: Plb1 | 2: Plc2, Plb5 | 4 | 5 |
| Sap proteins | 4: Sap5, Sap7, Sap 9, Sap10. | 1: Sap3 | 4: Sap2, Sap4, Sap6, Sap8 | 6 | 8 |
| Als proteins | 1: Als3 | 1: Als2 | 1: Als1 | 2 | 2 |
| Proteins with a role in virulence according to CGD | 12: Mnt1, Phr1, Het1, BglII, Rbt4, Rbt1, Asc1, Cdc42, Mnt2, Hex1, Kex2, Cat1 | 0 | 56: Hsp104, Nag1, Cdc3, Fas2, Vps21, Hxk1, Srv2, Rsr1, Mts1, Ras1, Tps1, Tps2, Alo1, Rvs161, Cdc10, Dac1, Arp2, Lap3, Kre5, Hsp21, Icl1, Ade5, Yhb1, Ade2, Gna1, Tpk2, Ftr1, Ssd1, Cka2, Mkc1, Orf19.3045, Ptc2, Met2, Nce103, Vtc4, Cmp1, Erg3, Nag6, Ura3, Slk19, Vps4, Orf19.3175, Och1, Ypt72, Csh3, Cek1, Pmt1, Pmt4, Bem1, Tcc1, Ssn6, Spf1, Pde2, Cla4, Mlt1, Spa2 | 12 | 68 |
a
ORF, open reading frame.
b
The protein is a translation elongation factor.
c
The protein is a translation release factor.
d
The protein is a tRNA synthetase or ligase.