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. 2022 May 10;10(3):e00916-22. doi: 10.1128/spectrum.00916-22

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Copyright © 2022 Phillips et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — (A) Killing by differentiated neutrophil-like HL-60 cells with 10% (vol/vol) complement after 1 h. SpnIII A failed to reach 50% killing at maximum neutrophil:CFU ratio (400:1) after 1 h. SpnIII B and SpnIII C reached 50% killing at 50 and 200 neutrophil:CFU ratios, respectively. Opsonophagocytic killing assays using antisera against (B) PiuA and (C) CbpA at 1 in 20 dilution. Effects of antibody-mediated killing were masked by large differences in antibody-independent killing (A), independent of differences in antisera target. Western Blot showing expression of antisera targets in locked SpnIII alleles included for comparison. MOI for assays presented in Fig. S1. *, P < 0.05; NSD, no significant difference.