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. 2022 Jun 1;10(3):e01054-22. doi: 10.1128/spectrum.01054-22

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FIG 1 — Comparison of multiplex bead assay results to results from two single antigen-based SARS-CoV-2 antibody assays. (A) The nucleocapsid protein (NP) antigen results from the multiplex assay were compared to those from the Abbott SARS-CoV-2 IgG assay. (B) Receptor-binding domain (RBD) antigen results from the multiplex assay were compared to those from the Ortho VITROS Anti-SARS-CoV-2 Total assay. Black dashed lines represent assay cutoffs: median fluorescence intensity (MFI) ratio of 1.2 for the multiplex assay, index of stored calibrator luminescent signal divided by a stored calibrator luminescent signal (S/C) of 1.4 for Abbott, and specimen luminescent signal divided by the luminescent signal at cutoff (S/C) of 1.0 for Ortho. Power function trendlines (red dashed lines) are shown with R² values. Unfilled data points represent specimens with discrepant results between the two assays.