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. 2022 May 11;7(3):e00312-22. doi: 10.1128/msystems.00312-22

TABLE 1.

Summary of in vitro and in vivo trophic measurements

Consumera Diet typea TDFglu-pheb TPexpectedb TPglu-pheb
In vitro growth conditions
 None Yeast-soy lipid agar (YE-YS) NA 1.0 1.0
 Xenorhabdus Yeast-soy broth (YE-YS) 6.53 2.0 1.9
 Steinernema (adults) Xenorhabdus on yeast-soy lipid agar (YE-YS) 6.96 3.0 2.9
 Steinernema (IJs) Xenorhabdus on yeast-soy lipid agar (YE-YS) 8.02 3.0 3.0
In vivo growth conditions
 None Galleria (base of food chain) NA 2.0 2.2
 None Galleria + PBS buffer (positive control) NA 2.0 2.2
 None Yeast-soy broth (YE-YS) NA 2.0 2.2
 Xenorhabdus (measured as Galleria-Xenorhabdus complex) Galleria NA 2.5 2.5
 Steinernema IJs Galleria-Xenorhabdus complex NA 3.5 4.6
a

Each organism in the ecosystem was assessed for its trophic position as a consumer under controlled in vitro conditions or in vivo within an insect cadaver. None indicates that the condition tested was diet only, no consumer.

b

Individual trophic discrimination factors (TDF), expected trophic positions (TPexpected), and measured TP (TPglu-phe) are given for each consumer diet. NA, not applicable.