TABLE 2.

Qualitative growth of X. nematophila lrhA mutant on minimal and defined media^a

Strain	Growth on:
	MMc	MM + 0.5% glycerolc	MM + 0.35% NaNH4PO4c	MM + Asp, Glu, Leud	MM + 0.1% Casamino Acidse
Wild type	+	+	+	+	+
lrhA1::Tn10	−	−	−	+/−	+
lrhA1::Tn10+lrhAb	+	+	+	+	+

^aMM, minimal medium based on reference 85; see Materials and Methods. +, growth was indistinguishable from wild type; −, the activity was not detected in the specified time frame.

^blrhA1::Tn10 mutant containing a wild type copy of lrhA at the attTn7 site of the chromosome.

^c+, wild-type growth detected after 48 h. −, little to no growth detected after 72h.

^d+, wild-type growth detected after 48 h. +/−, some growth detected after 72 h. Asp, 0.25% aspartate; Glu, 0.01% glutamate; Leu, 0.01% leucine.

^e+, wild-type growth detected after 24 h.