TABLE 3.
Steinernema carpocapsae homologs of Caenorhabditis elegans kynurenine pathway enzymes
| Predicted protein producta | Genea | C. elegans accession no. | S. carpocapsaeb accession no. | Log2 fold change, head vs tail (BH adjusted P value)c | Alignment statisticsd |
|||
|---|---|---|---|---|---|---|---|---|
| E value | Id | Pos | Gap | |||||
| Tryptophan 2,3-dioxygenase | tdo-2 | NP_498284.1 | TKR75863.1, L596_017097 | −1.23 (0.43) | 1e−165 | 228/343 (66%) | 271/343 (79%) | 1/343 (0%) |
| Abhydrolase_3 domain-containing protein | afmd-1 | NP_501149.1 | TKR61092.1, L596_028252 | 0.23 (0.79) | 2e−27 | 79/219 (36%) | 115/219 (52%) | 11/219 (5%) |
| Kynurenine 3-monooxygenase | kmo-1 | NP_506025.1 | TKR66772.1, L596_023014 | −1.67 (0.035) | 0.0 | 289/449 (64%) | 341/449 (75%) | 15/449 (3%) |
| Kynureninasee | flu-2 | Q18026.1 | TKR58414.1, L596_029862 | -2.57 (0.0097) | 0.0 | 276/472 (58%) | 344/472 (72%) | 11/472 (2%) |
| Kynureninasee | kynu-1 | NP_509023.1 | TKR82837.1, L596_016512 | 7.37 (0.0019) | 0.0 | 254/452 (56%) | 329/452 (72%) | 5/452 (1%) |
| 3-Hydroxyanthranilate 3,4-dioxygenase | haao-1 | NP_505450.1 | TKR95465.1, L596_009631 | −1.13 (0.35) | 5e−58 | 89/196 (45%) | 133/196 (67%) | 1/196 (0%) |
| Uridine 5′-monophosphate synthase | umps-1 | G5EDZ2.1 | TKR60155.1, L596_027451 | 0.85 (0.37) | 3e−104 | 188/484 (39%) | 282/484 (58%) | 48/484 (9%) |
| Kynurenineto-oxoglutarate transaminase | nkat-1 | NP_510355 | TKR80742.1, L596_014762 | −1.57 (0.053) | 5e−166 | 232/422 (55%) | 305/422 (72%) | 4/422 (0%) |
Based on pathway descriptions provided by Hyland et al. (90) and McReynolds et al. (55) and KEGG database analysis (91).
Reciprocal BLASTp hits with indicated Caenorhabditis elegans protein based on genome sequence reported in Rougon-Cardoso et al. (92). Accession numbers and locus tags are provided.
From Rodriguez et al. (75). BH: Benjamini-Hochberg Procedure.
Statistics derived from NCBI BLASTp server (93). Id: fraction and percent identical amino acids in aligned region. Pos: fraction and percent similar amino acids in aligned region. Gap: fraction of sites within aligned region in which gaps were introduced to maintain alignment.
Two kynureninase homologs were identified in the S. carpocapsae predicted proteome and are 62.6% identical across the length of the protein. Both encode the conserved active site lysine (94).