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. 2022 Apr 18;10(3):e00595-22. doi: 10.1128/spectrum.00595-22

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Copyright © 2022 Dubey et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — AeCullin-3 is proviral during CHIKV replication in Aag2 cells. (a) MTT assay to determine percentage of viable cells in the presence of MG132 at 0.1 μM, 1 μM, and 10 μM. One percent DMSO was used as vehicle control. (b) Plaque assay for viral titer determination in MG132 - CHIKV pretreatment assay. (c) qRT-PCR for CHIKV genomic RNA levels in MG132-CHIKV pretreatment assay. (d) Plaque assay for viral titer determination in MG132-CHIKV post-treatment assay. (e) qRT-PCR for CHIKV genomic RNA levels in MG132-CHIKV posttreatment assay. Rps17 was used as an endogenous control in qRT-PCR, and the results were analyzed using 2^−ΔΔ^CT. The experiments were performed a minimum of three times, with each experiment set in triplicates. Data are expressed as mean ± SD. ****, P value < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.1 (versus control).