FIG 2.

Intracellular replication and virulence tests of Δsbe1 mutant. A. The effect of SBE1 deletion on the replication ability of tachyzoites in different carbon source mediums, and intracellular replication rates were determined by the fraction of vacuoles containing 1, 2, 4, 8, and 16 or more parasites. B. Survival curves of mice infected with tachyzoites of indicated strains. ME49 and Δsbe1 strains were respectively used to infect ICR mice (100 parasites per mouse, 10 mice for each group) by intraperitoneal injection, and the survival of mice was monitored for 30 days. C. Brain cysts of ICR mice infected with corresponding strains. The brain cysts of positive mice that survived 30 days after infection with the indicated strains were collected and stained with DBA-FITC, and the number of brain cysts was counted under a microscope. ****, P < 0.0001, ns represents not significant, two-way ANOVA, Mantel-Cox log-rank test. Data represents mean ± SEM of three independent experiments.