FIG 5.

Growth and virulence of Δα-amyΔsbe1 mutant in vitro. A. Plaque assay of parasites in vitro. Purified indicated tachyzoites were used to infect HFF cells, and 200 parasites were cultured for 14 days. B. Relative size of plaques in Fig. 5A C. Intracellular replication rates of parasites in vitro. Freshly egressed tachyzoites of indicated strains were used to invade HFF monolayer for 1 h, and then the parasites were grown for another 24 h. Parasite replication was determined in the same way as above. D. Survival curves of mice infected with tachyzoites of indicated strains. **, P < 0.01; *, P < 0.05, ns represents not significant, one-way ANOVA, two-way ANOVA, Mantel-Cox log-rank test. Data represent mean ± SEM of three independent experiments.