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. 2022 Apr 21;10(3):e01891-21. doi: 10.1128/spectrum.01891-21

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Copyright © 2022 Yang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — Starch branching enzyme 1 (SBE1) is important for replication of bradyzoites in vitro. A. Detection of the conversion rates of bradyzoites, statistics of the proportion of Dolichos biflorus agglutinin (DBA)-positive parasitophorous vacuoles (PVs) in all indicated parasites after 4 days of induction under alkaline culture conditions. B. Parasite replication under bradyzoite-inducing conditions. The indicated parasites were induced under alkaline culture conditions for 4 days and then grown under alkaline conditions for an additional 36 h. Parasite replication was determined in the same way as above. C. Detection of SBE1 deletion mutant on the ability of bradyzoite reactivation to tachyzoites in vitro. The indicated strain was first cultured under alkaline culture conditions for 15 days to form bradyzoites (DBA-positive) and then changed to grow in tachyzoite culture conditions for 48 h. The samples were stained with DBA-FITC and a reduction of DBA-positive vacuoles means that bradyzoites were transformed into tachyzoites. **P < 0.01, ns represents not significant, one-way ANOVA, two-way ANOVA. Data represent mean ± SEM of three independent experiments.