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. 2022 Apr 21;10(3):e01891-21. doi: 10.1128/spectrum.01891-21

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Copyright © 2022 Yang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 7 — Effects of SBE1 deficiency on the morphology and function of cysts. A. Brain cysts of ICR mice infected with corresponding strains. The brain cysts of positive mice that survived 30 days after infection with the indicated strains were collected and stained with DBA-FITC, and the number of brain cysts was counted under a microscope. B. Detection of amylopectin accumulation in the brain cysts by IFA-PAS staining. The brain cysts were purified and then the amylopectin accumulation was detected with IFA-PAS staining. C. Relative sizes of brain cysts obtained in Fig. 7A. D. Brain cyst virulence of indicated strains. A total of 20 brain cysts were administered intragastrically per mouse (5 mice for ME49 and Δα-amyΔsbe1 group, and 4 mice for Δα-amy and Δsbe1 group), and the survival rate was monitored for 30 days. ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05, one-way ANOVA, Mantel-Cox log-rank test.