FIG 1.

(A) Bacteriocytes stained with DAPI. Tubular Carsonella cells were squeezed out by applying gentle pressure on a coverslip. Extremely long Carsonella cells surrounding host nuclei show strong DAPI signals. Within Carsonella cells, numerous spots with higher signal intensities are observed. Bar, 10 μm. (B) Quantitative PCR analysis of Carsonella genomic copy number in the bacteriome of Pachypsylla venusta. Abundance values of the Carsonella 16S rRNA gene were normalized to the psyllid nuclear genes for RpL18 (left) and RpL32 (right). All data points (female, magenta; male, cyan) of six biological replicates of each sex are presented. Black dots and black bars represent means and standard deviations, respectively.