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Sensitivity of qPCR probesets on DNA extracted from spiked human bile samples. Human bile samples were spiked with varying concentrations of S. Typhi strain Ty2. DNA was extracted from bile samples using the optimized protocol, and qPCR was completed using either the oriC probeset (A), the STY0201 probeset (B), or the stoD probeset (C). Data are shown as means ± SDs from duplicate technical replicates.
