TABLE 3.

Detection of S. Typhi from antibiotic spiked bile by culture and qPCR using the STY0201 probeset

	Cephalosporin (50 μg mL−1)a
Concn of S. Typhi	Cefazolin		Cefotaxime		Ceftriaxone
	Culture (dilution)	qPCR (Cq)b	Culture	qPCR (Cq)	Culture	qPCR (Cq)
102 CFU mL−1	+ (1 in 100)	37.0	NDc	37.3	ND	38.1
103 CFU mL−1	+ (1 in 50)	33.1	−	34.1	−	33.5
104 CFU mL−1	+ (1 in 50)	30.3	−	30.5	−	30.5
105 CFU mL−1	ND	ND	−	26.7	−	26.7

^a+, growth; −, no growth.

^bCq, quantification cycle.

^cND, not determined.