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. 2022 Apr 18;10(3):e00644-22. doi: 10.1128/spectrum.00644-22

FIG 2.

FIG 2

Effect of the genes encoding putrescine and spermidine biosynthesis on expression of T3SS exsCEBA genes. (A) Expression patterns of exsCEBA-lacZ in wild-type strain PAO1 and the mutants of putrescine biosynthesis associated genes. (B) Expression patterns of exsCEBA-lacZ in wild-type strain PAO1 and the mutants of spermidine biosynthesis associated genes. (C) Expression patterns of exsCEBA-lacZ in wild-type strain PAO1, spermidine biosynthesis gene mutants, and corresponding complementation strains in MMN medium. Bacterial strains were cultured in different growth media as indicated. The experiment was repeated at least triplicates in each assay and error bars indicate standard deviations. Statistics significance: **, P < 0.01; ***, P < 0.001. Statistical analysis compared to wild-type strain PAO1 in the same medium was performed by using Student's t test.