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. 2022 Apr 18;10(3):e00644-22. doi: 10.1128/spectrum.00644-22

FIG 3.

FIG 3

Effect of the genes encoding polyamine transportation on expression of T3SS exsCEBA genes. (A) Impact of spuE-potD double deletion and with corresponding overexpression on exsCEBA expression. (B) Impact of spuE-speE double deletion and with corresponding overexpression on exsCEBA expression. Wild-type strain PAO1 and its derivatives were cultured in different growth media as indicated. Experiment was repeated at least triplicates in each assay and error bars indicate standard deviations. Statistics significance: ns, no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001. Statistical analysis compared to wild-type strain PAO1 or spuE-potD double deletion mutant in the same medium was performed by using Student’s t test.