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. 2022 Apr 18;10(3):e00644-22. doi: 10.1128/spectrum.00644-22

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Copyright © 2022 Lin et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — Spermidine biosynthesis is essential for the full virulence of Pseudomonas aeruginosa. (A) Relative LDH released from the cell line A549 challenged with wild-type strain PAO1 and its derivatives at different time points postinoculation. (B) In trans expression of speE and spuE in corresponding mutants restored the cell cytotoxicity. Wild-type strain PAO1 and its derivatives were grown in MMN medium, and the LDH activity was measured 4-h postinoculation. Experiment was repeated at least triplicates in each assay and error bars indicate standard deviations. Statistics significance: *, P < 0.05; **, P < 0.01; ***, P < 0.001. Statistical analysis compared to wild-type strain PAO1 in the same incubation time was performed by using Student’s t test.