FIG 7.

Effect of spermidine biosynthesis and transportation on the expression of T3SS associated protein. Immunoblotting detection of the protein levels of ExsA, ExoS, and PcrV in different strains cultured in LBN medium (A) or MMN medium (B). The cell extracts were subjected to SDS-PAGE separation and for immuno-blotting. RNA polymerase (RNAP) was used as an internal control. Symbol: ECP, extra-cellular proteins; ICP, intra-cellular proteins; + and −, presence (+) or absence (−) of the expression construct in corresponding bacterial strain.