FIG 9.

Expression patterns of spermidine-related genes. (A) Time-course analysis of spermidine-related gene expression in wild-type strain PAO1 by RT-qPCR. Total RNAs were collected when bacterial cells were grown to the corresponding OD₆₀₀. (B) Impact of speE null mutation on expression of spermidine-related genes. Total RNAs were collected when bacterial cells were grown to an OD₆₀₀ of 1.2. The rpoD gene was used as an internal control. Each assay was conducted with at least triplicates and error bars indicate standard deviations. Statistics significance: ns, no significance; **, P < 0.01. Statistical analysis comparison to wild-type strain PAO1 was performed by using Student’s t test.