FIG 1.
(A) Spotting assay to evaluate stress response changes associated with GCN5 deletion in C. glabrata. Five microliters of 10-fold serial dilutions of the indicated cells was spotted onto plain YPD plates and YPD plates supplemented with hydrogen peroxide (H2O2) (10 mM), SDS (0.01%), calcofluor white stain (10 μg/mL), Congo red (50 μg/mL), fluconazole (16 μg/mL), caspofungin (0.03 μg/mL), and micafungin (0.008 μg/mL). Plates were incubated at 37°C for 24 h. (B) Spotting assay with fks mutants (Fks1-625delF, Fks1-S629P, Fks2-659delF, and Fks2-S663P) with or without Gcn5 function to assess the impact of GCN5 on echinocandin resistance. Micafungin was tested at 0.03 μg/mL, and caspofungin was tested at 0.25 μg/mL.