TABLE 3.

Deletion of GCN5 leads to decreases in frequencies of resistant colonies and resistance-conferring mutations

Antifungal agent	Concn (μg/mL)	Strain	Time (h)	Avg resistant colony frequency ± SD	No. of mutations identified/total no. of colonies sequenceda	Protein mutation (nucleotide change)
Micafungin	0.03	WT	24	1.32E−07 ± 5.09E−09	5/5	Fks2-659delF (1971_1973delTTC)
			48	1.32E−07 ± 1.94E−08	12/12	Fks2-659delF (1971_1973delTTC)
	0.03	gcn5Δ	24	0
			48	0
	0.008	WT	24	1.57E−08 ± 4.32E−09	NT
			48	1.73E−08 ± 1.64E−10	NT
	0.008	gcn5Δ	24	0
			48	2.23E−08 ± 2.23E−08	4/4	Fks2-659delF (1971_1973delTTC)
Fluconazole	16	WT	24	3.95E−04 ± 7.56E−06	NT
			48	6.72E−05 ± 1.29E−06	NT
	16	gcn5Δ	24	2.52E−04 ± 1.71E−05	NT
			48	3.90E−05 ± 2.64E−06	NT
	64	WT	24	2.99E−03 ± 5.73E−05	NT
			48	1.60E−03 ± 1.34E−03	NT
	64	gcn5Δ	24	2.84E−03 ± 1.68E−04	NT
			48	9.33E−05 ± 8.09E−05	NT
	256	WT	24	2.67E−02 ± 5.12E−04	0/2	No PDR1 mutation identified
			48	3.98E−02 ± 7.62E−04	0/1
	256	gcn5Δ	24	4.26E−03 ± 2.88E−04	0/1
			48	8.95E−03 ± 8.77E−03	0/1
	1,024	WT	24	2.08E−03 ± 3.98E−05	0/1
			48	2.26E−01 ± 7.22E−02	0/2
	1,024	gcn5Δ	24	0
			48	1.37E−04 ± 9.28E−06	0/2

^aNT, not tested.