FIG 1.

The prrT/prrA gene pair act as a type II TA system. (A) Deletion of prrA in 39016 strain revealed a decrease in the growth rate; the growth curve of the mutants in comparison to the WT, and complementation by arabinose induced prrA expression in the mutant. (B) prrT expression is toxic only for the ΔprrA strain; the growth curve of prrT induced OE in the WT and mutant strains, and the inducer was added immediately after the dilution. (C) prrT/prrA gene pair expressed as a polycistron; the following sets of primers were used for the operon verification: (a) 500 bp upstream to prrT, (b) prrT_F, (c) prrT_R, (d) prrA_F, (e) prrA_R, and (f) 500 bp downstream to prrA. The cDNA results represent the transcripts, while gDNA results represent the bacterial genome as a positive control. (D) PrrT/PrrA form a protein complex together; BACTH assay on indicative LB plates containing IPTG and x-gal. The above (A) and (B) graphs are the averages of three independent experiments consisting of five replicates each. Error bars represent the standard deviations.