Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers used in this study
| Primers | Sequences (5′–3′) | Application |
|---|---|---|
| F1 | ACCAGGAGGGCCCGGTAAAAAC | Mutant clone |
| R1 | ACGGATCTCCTCCTTCGCCTCCTATGCCTGGTC | |
| F2 | GACCAGGCATAGGAGGCGAAGGAGGAGATCCGT | |
| R2 | CTGCCAGTCTCTGAGCTCCCTT | |
| JEV-C-F | GGCTTTTATCACGTTCTTCAAGTTT | Relative qPCR |
| JEV-C-R | TGCTTTCCATCGGCCTAAAA | |
| β-actin-F | CACTGCCGCATCCTCTTCCTCCC | |
| β-actin-R | CAATAGTGATGACCTGGCCGT | |
| JEV-E-F | TGGTTTCATGACCTCGCTCTC | Absolute qPCR |
| JEV-E-R | CCATGAGGAGTTCTCTGTTTCT | |
| Probe | CCTGGACGCCCCCTTCGAGCACAGCGT |
