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Hydrolysis of levan by recombinant rFruA and rFruB. (A) Hydrolysis of levan by recombinant rFruA and a mixture of rFruA/rFruB at different time points (0, 0.5, 1, 2 and 4 h). (B) Concentration of reducing sugar (μg/mL) was measured after hydrolysis of levan by 10 and 20 pmol of purified recombinant rFruB after 12, 24, and 30 h. Data are representative of three independent experiments performed at least in triplicate. (P < 0.05).
