FIG 4.

Thin Layer Chromatography (TLC) analysis of levan degradation. TLC at (A) 1 h, (B) 4 h and (C) 24 h time points. Lane 1 included a standard of fructose, sucrose and rafiinose; lane 2 0.5% untreated levan; lane 3 0.5% levan treated with BSA; lanes 4, 5, and 6 are levan treated with 2, 5, and 10 pmol of rFruA respectively; lanes 7, 8, and 9 were 0.5% levan treated with 10, 50, and 100 pmol of rFruB; and lanes 10, 11, and 12 were levan treated with a mixture of rFruA (2 pmol) with increasing concentrations of rFruB (10, 50 and 100 pmol). Data presented are from the same experiment and representative of multiple repeats across different days.