TABLE 1.
Strains and plasmid used in this studya
| Strains or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| S. mutans | ||
| UA159 | Wild-type | Laboratory stock (ATCC700610) |
| ΔfruA | fruA:: Kan | This study |
| ΔfruB | fruB:: Kan | This study |
| ΔfruAB | fruAB:: Kan, by introducing a polar kanamycin (Ω kan) mutation in fruAB operon by allelic exchange. | This study |
| ΔfruA::pIB184 | ΔfruA harboring pIB184, Emr | This study |
| ΔfruB::pIB184 | ΔfruB harboring pIB184, Emr | This study |
| ΔfruAB:pIB184 | ΔfruAB harboring pIB184, Emr | This study |
| ΔfruA::pIB184::fruB | ΔfruA harboring pIB184::fruB, Emr | This study |
| ΔfruB::pIB184:fruB | ΔfruB harboring pIB184:: fruB, Emr | This study |
| ΔfruAB::pIB184:fruB | ΔfruAB harboring pIB184:: fruB, Emr | This study |
| CAT01 | veg promoter and RBS of fruB transcriptionally fused with cat and integrated within UA159 chromosome via the vector pJL84, Kanr | This study |
| CAT02 | veg promoter and igr of fruAB operon transcriptionally fused with cat and integrated within UA159 chromosome via the vector pJL84, Kanr | This study |
| CAT03 | fruA promoter and RBS of fruB transcriptionally fused with cat and integrated within UA159 chromosome via the vector pJL84, Kanr | This study |
| CAT04 | fruA promoter and igr of fruAB operon transcriptionally fused with cat and integrated within UA159 chromosome via the vector pJL84, Kanr | This study |
| CAT05 | 3′ end of fruA and RBS of fruB transcriptionally fused with cat and integrated within UA159 chromosome via the vector pJL84, Kanr | This study |
| CAT06 | 3′ of fruA and igr of fruAB operon transcriptionally fused with cat and integrated within UA159 chromosome via the vector pJL84, Kanr | This study |
| CAT07 | igr of fruAB operon transcriptionally fused with cat and integrated within UA159 chromosome via the vector pJL84, Kanr | This study |
| Plasmids | ||
| pIB184 | Shuttle expression plasmid with constitutive P23 promoter, Emr | (34) |
a
Kanr, resistant to Kanamycin; Emr, resistant to erythromycin. Constructs containing the cat gene were synthesized using GBlocks (Integrated DNA Technologies, IA, USA).