TABLE 3.
Primers used in this study
| Primers | Sequences (5′–3′) |
|---|---|
| fruA-1000 up-S | TTATTATTGGACAAGCAGCGCTGGATAG |
| fruA-1000 up-AS | TTCCTCTTTTTCCTTGCTCTCCTTTTCAAATTTATGAAAC |
| NpKan-fruA-S | AGCAAGGAAAAAGAGGAAGGAAATAATAAATGGC |
| NpKan-fruA-AS | GTTTACTTTAACTAAAACAATTCATCCAGTAAAATATAATATTTTATTTTC |
| fruA-1000 down-S | ATTGTTTTAGTTAAAGTAAACCTCTAGATTTTGACXG |
| fruA-1000 down-AS | CATCCAAGCTATACTCTTAACGACG |
| fruB-1000 up-S | GATCAATACCACCATATTAAAGTCAC |
| fruB-1000 up-AS | TTCCTCTTTAAGACACCTCTATCTATTGTTAGCC |
| NpKan-fruB-S | AGGTGTCTTAAAGAGGAAGGAAATAATAAATGGC |
| NpKan-fruB-AS | TTATTGCTGCTAAAACAATTCATCCAGTAAAATATAATATTTTATTTTC |
| fruB-1000 down-S | ATTGTTTTAGCAGCAATAAAACTAAGTCTTAAGTCG |
| fruB-1000 down-AS | TATCAGCAACTTGAACTCGATGAAG |
| fruBBamH1pIB184-S | CGCGGATCCTCTAGATTTAAGGAGATATACATATGCTAAAGAAAAAATTAG |
| fruBXho1pIB184-AS | CCGCTCGAGTTACGTTTCCAAATTAGC |
| fruA-RT_S | CGACTAGTACGGATTTGATTCACTGG |
| fruA-RT-AS | CCACCCTTAGCCGTCTTAAATAAGC |
| fruB-RT_S | AGGTTAATGATGTACCTAGCTGAGGG |
| fruB-RT-AS | CCACAAGACCTAAGTCTTTTCCCC |
| luxS-RT_S | ACTGTTCCCCTTTTGGCTGTC |
| luxS-RT-AS | AACTTGCTTTGATGACTGTGGC |
| divIB-RT_S | AATGGTACCCGTGTTGATACC |
| divIB-RT-AS | GAGGTTCGAGAACTGGTTCAA |
| dpr-RT_S | GTGGTTCAGGCTTCCTTTATCT |
| dpr-RT-AS | TTGATTACTATCGGTGGCGC |
| gyrA-RT_S | CCAAGAATCTGCTGTCCG |
| gyrA-RT-AS | TTGCGACTATCTGCTATGTG |