FIG. 5.

In situ detection of Eikelboom type 021N bacteria in activated sludge from the WWTP Großlappen (Munich, Germany). (A) An interference contrast image of a sludge floc. (B) Fluorescence in situ hybridization of the same microscopic field using probes G2M (labeled with Cy3, colored in green) and G123T (labeled with Cy5, colored in red). Yellow filaments did hybridize with both probes and were thus identified as members of group II. Red filaments (arrow) only bound probe G123T and are most likely members of the genus Thiothrix. Bar, 20 μm.