TABLE 6.

Difference alignment of the target region of the 16S rRNA of Eikelboom type 021N isolates for various probes

Probe and organism or target	Target sequencea
G1B
Target sequence (5′-3′)	GCAAGGAACUCGAACACA
Type 021N group I	------------------
Type 021N group II	UUCG-----G-U------
Type 021N group III	---------CU-C-----
T. nivea	UUCG-----A-UG-----
T. unzii	UUCG-----A-U------
T. ramosa	UUCG-----A-U------
T. fructosivorans I	UUCG-----A-U------
T. fructosivorans Q	UUCG-----A-U------
T. defluvii	---------CUAC-----
G2M
Target sequence (5′-3′)	CUAAGGGGUCGGUGGUGC
Type 021N group II	------------------
Type 021N group I	------A-----------
Type 021N group III	U-U---AU--A-------
T. nivea	U-U----U-U--------
T. unzii	UCU--A------------
T. ramosa	U-U----U-U-------G
T. fructosivorans I	U-U--A-U-U-------G
T. fructosivorans Q	U-U--A-U-U-------G
T. defluvii	--U--AAU----------
G3M
Target sequence (5′-3′)	AUGGCAGGAAUCCCUGAG
Type 021N group III	------------------
Type 021N group I	---U-GA-------GU--
Type 021N group IIb	---UAGC-------GC--
T. eikelboomii AP3	GA----C---AG-G--G-
T. nivea	UC--A------A--A-U-
T. unzii	UC--A-----CA--A-U-
T. ramosa	GA----C---AG-G--G-
T. fructosivorans I	G---AGCA------A--A
T. fructosivorans Q	GA----C---AG-G--G-
T. defluvii	----U-------------
G123T
Target sequence (5′-3′)	UGCAUAGAGAUCGGAAGG
Type 021N group I	------------------
Type 021N group II	------------------
Type 021N group III	------------------
T. nivea	------------------
T. unzii	------------------
T. ramosa	------------------
T. fructosivorans I	------------------
T. fructosivorans Q	------------------
T. defluvii	------------------

^a–, identical to the target sequence. 

^bExcept T. eikelboomii AP3.