FIG 3.

dSTORM imaging of an antibody-specific protease in Mycoplasma mycoides subsp. capri. M. mycoides subsp. capri 0582-HA cells expressing an HA tag-fused variant of the serine protease MIP₈₂ were immunolabeled and imaged by dSTORM. The tagged fusion protein is expressed from the native genomic locus and replaces the wild-type variant. The data presented here correspond to a single representative field of view (512 by 512 pixels; pixel size = 160 nm). (A) Sample images of M. mycoides subsp. capri 0582-HA cells. For each field of view, the images correspond to epifluorescence (diffraction limited) (left), superresolved reconstruction (40 nm pixel) (middle), and Tesseler segmentation (right). Scale bar = 1 μm. (B) Tesseler clustering of the fluorescence signal. For each field of view, the number of clusters per Tesseler-segmented object was computed. The bar graphs display the distribution of the numbers of clusters per object. (C) Object and cluster sizes. The dot plot presents the area (in nm²) of each object and cluster segmented by Tesseler, to which a boxplot showing the median, interquartile range, minimum, and maximum values is overlaid. The median value of each data set is indicated.