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. 2022 Jun 6;10(3):e00125-22. doi: 10.1128/spectrum.00125-22

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Copyright © 2022 Fang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — IPA increased survival and decreased serum levels of proinflammatory cytokines in mice after cecal ligation and puncture (CLP) operation. The serum levels of procalcitonin (A), lipopolysaccharide (B), and creatinine (C) were measured using enzyme-linked immunosorbent assay (ELISA) in the sham-operated (CON) and CLP-operated mice. (D, E) Mice who survived 24 h after surgery were subjected to open field test to assess their motor ability and anxiety behavior. The traveling speed (D) and the ratio of center distance to total distance (E) were collected and analyzed. (F) Survival rate. The serum levels of IL-1β (G) and TNF-α (H) were measured using ELISA in CON, IPA-treated, and saline-treated mice. n = 6 to 20; *, P < 0.05; **, P < 0.01; ***, P < 0.001.