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. 2022 May 24;10(3):e00681-22. doi: 10.1128/spectrum.00681-22

FIG 1.

FIG 1

Tumor suppressor p53 expression is conserved in HPV16 immortalized cell lines and patient derived xenografts. (A) Western blot analysis of p53 and pRb in N/tert-1 cells stably expressing the HPV16 genome (lane 2), HPV16 E6+E7 (lane 4) or empty vector (lane 1) compared to Human tonsillar keratinocytes immortalized with HPV16 (lane 3). Two independent human foreskin keratinocyte (HFK) donors were immortalized with wild-type HPV16 (lanes 6 and 7) or exogenous expression of the HPV16 E6 and E7 oncogenes (lane 8, cells from donor 1). (B) Western blot analysis of p53 expression in 4 HPV-negative and 4 HPV-positive patient derived xenografts. (C) p53 expression following 24-h cisplatin treatment of primary HPV-positive head and neck cancer cell lines (SCC47 and SCC104) DMF solvent only was used as drug-free control. All Western blots utilized GAPDH as an internal loading control.