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. 2022 Jun 8;10(3):e00006-22. doi: 10.1128/spectrum.00006-22

FIG 1.

FIG 1

Expression of H19 and p53 in Epstein-Barr virus (EBV)-positive and EBV-negative tumor cell lines. (A) The expression of H19 mRNA in EBV-positive tumor cell lines (GT38, GT39, SNU719, and C666) and EBV-negative tumor cell lines (SGC7901, AGS, BGC823, MKN45, HGC27, CNE, and HONE) were measured by quantitative (q)RT-PCR. (B) Western blot analysis showing the protein levels of latent membrane protein 1 (LMP1) or p53 in EBV-positive tumor cell lines (GT38, GT39, SNU719, and C666) or EBV-negative tumor cell lines (SGC7901, AGS, BGC823, CNE, and HONE). (C) The expression of H19 mRNA in LMP1-transfected SGC7901 and CNE cells. (D) The expression of p53 protein in LMP1-transfected SGC7901 and CNE cells. (E) The expression of p53 protein in SGC7901 transfected with LMP2A and EBER1. (F) Fluorescence efficiency of CNE cells transfected with vector and LMP1 plasmids. NC, negative control cell. Original magnification × 400 for A to F. All experiments were performed with at least 3 replicates. *, P < 0.05, **, P < 0.01.