FIG 2.

EBOV strains bearing variants of GPs that elicit enhanced infectivity are enhanced by TIM-1 expression. (A) Western blots of lentiviral particles pseudotyped with variant EBOV GPs probed with mab114. The ratio of GP to p24 (GP: p24) was calculated by the Chemiscope 6000 analysis system. In panels B, C, D, and E, the GPs present on virions used here were normalized according to the results of Western blots. (B) Cell entry of the 293T cells by EBOV and EBOV bearing variant GPs. Data points represent independent experiments with three independent viral stocks; each data point represents a normalized transduction. Data are the fold increase of viral entry into TIM-1-expressing (C), Axl-expressing (D), DC-SIGN-expressing (E), and LSECtin-expressing 293T cells (F) compared with vector-expressing 293T cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001.