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. 2022 Apr 6;10(3):e02212-21. doi: 10.1128/spectrum.02212-21

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Copyright © 2022 Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Identification functional properties of TIM-1 protein. TIM-1 proteins inhibit Ebola virus cell entry. (A) Neutralization of EBOV, BDBV, and SUDV by dilutions of TIM-1 ECD. (B) TIM-1 ECD competes for EBOV entry to 293T cells that were transfected with polymorphic TIM-1. (C) TIM-1 ECD competes for EBOV and mutated EBOV cell entry. (D) Function of TIM-1 ECD, TIM-1 IgV, and TIM-1 ΔIgV protein to compete for EBOV entry. (E) Binding affinity of recombinant TIM-1 ECD, TIM-1 IgV, and TIM-1 ΔIgV to PS and PC. *, P < 0.05; **, P < 0.01.