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. 2022 Apr 6;10(3):e02212-21. doi: 10.1128/spectrum.02212-21

FIG 4.

FIG 4

Inhibition of EBOV infection by TIM-1 ECD in different cell lines. (Left panel) Flow cytometric detection of TIM-1, Axl, and DC-SIGN expression on the surface of Huh7.5.1 cells (A), A549 cells (B), Axl-expressing 293T cells (C), TIM-1-expressing 293T cells (D), DC-SIGN-expressing cells (E), and 293T cells (G) using PE-conjugated anti-TIM, PE-conjugated anti-Axl, and APC-conjugated anti-DC-SIGN antibodies. The expression level of LSECtin in LSECtin-expressing 293T cells (F) was detected by RT-qPCR. (Right panel) TIM-1 ECD protein impeded EBOV entry into Huh7.5.1 cells (A), A549 cells (B), Axl-expressing 293T cells (C), TIM-1-expressing 293T cells (D), DC-SIGN-expressing cells (E), LSECtin-expressing 293T cells (F), and 293T cells (G). *, P < 0.05; **, P < 0.01; ***, P < 0.001.