FIG 5.

Analysis of synergistic effects of TIM-1 protein and ADI-15946 on impeding cell entry of virus. The inhibition was evaluated by varying one substrate at a fixed concentration of another. (A, B, C) Cooperative function of TIM-1 protein and ADI-15946 on impeding cell entry of EBOV (A), BDBV (B), SUDV (C) when the doses of TIM-1 ECD were titrated. (D, E, and F) Cooperative function when the doses of ADI-15946 were titrated. (G) TIM-1 ECD inhibits EBOV attachment, but ADI-15946 does not. EBOV pseudovirus was incubated with TIM-1 ECD (left panel) or ADI-15946 (right panel) at 37°C for 1 h before being added to TIM-1-expressing cells. Then, the cell-virus blend was transferred to 4°C for 30 min to allow virus attachment, and cells were washed with PBS three times. Total RNA was extracted and used for quantification of EBOV GP by RT-qPCR. *, P < 0.05; **, P < 0.01; ***, P < 0.001.