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. 2022 May 23;10(3):e01106-22. doi: 10.1128/spectrum.01106-22

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Copyright © 2022 Yang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — Homology and reverse complementarity sequence statistics. (A) The percentage of homology and reverse complementarity sequences in the simulated circRNAs and experimentally confirmed HCMV circRNAs. (B) RNA structure prediction for HCMV genomic region FJ616285.1:29458|30121. (C) Illustration of divergent primers selectively amplify circular form circRNA number 5, while convergent primers amplify both linear and circular form circRNA number 5. (D) Inverse RT-PCR result of 293T cells transfected with the regions FJ616285.1:29489|30092 and FJ616285.1:29458|30121 linear form expression vector. (E) Representative Sanger sequencing results were shown in (D). BSJ breakpoints are indicated by dashed lines. Donor (green), acceptor (blue) sequences, and downstream/upstream sequences (gray) flanking the junction were aligned to the BSJ sequence.