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. 2022 May 16;10(3):e01927-21. doi: 10.1128/spectrum.01927-21

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Copyright © 2022 Martínez-Figueroa et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 1 — Examples of the usefulness of the A-S dye solution in the diagnosis of BV. (A and B) Fresh observation of a normal squamous epithelial cell (A) and clue cells (B) in a nonstained sample. (C to E) Normal squamous epithelial cells (with normal morphology) and examples of clue cells in samples from patients with BV using the A-S dye solution. (F) Bacterial morphotypes attached to the surface of epithelial cells, including curved bacilli (red arrow) and a coccobacillus (black arrow). (G) Leukocytes (polymorphonuclear). (H) Presumptive koilocyte (associated with HPV infection) with karyomegaly and perinuclear halo (yellow arrow). (I) Group of squamous epithelial cells with inflammation-related changes such as karyomegaly (green arrow) and perinuclear halo (yellow arrow) during Candida infection (blue arrow) (1000X amplification).